DNAPLOT — Immunogenetic Alignment & Junction Analysis Portal

⚙️ Sequence Submission & Target Configuration

Species Target

Receptor / Chain Locus

Display Width (bp / line)

Landmark Presets:

📖 Sequence Format Guidelines & Physiological Boundary Invariants: Choose format below or click 'Insert Example' to test immediately

Nucleotide DNA: Provide variable domain in any reading frame (the engine automatically detects the correct productive reading frame and mature FR1 anchor). Variable domain terminates strictly at canonical J-segment boundary (`TVSS`/`TVSA` or `VEIK`/`LEIK`/`TVL`).

>humIGHV3-23_VH Trastuzumab_Reference
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAACATCAAAGACACCTATATACACTGGGTGCGTCAGGCCCCGGGTAAGGGCCTGGAATGGGTTGCAAGGATTTATCCTACGAATGGTTATACTAGATATGCCGATAGCGTCAAGGGCCGTTTCACCATAAGCGCAGACACATCCAAAAACACAGCCTACCTGCAGATGAACAGCCTGAGAGCCGAGGACACGGCCGTGTATTACTGTTCGAGATGGGGAGGGGACGGCTTCTATGCTATGGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCA

Protein Amino Acid: Standard IUPAC single-letter amino acid code. Variable domains are automatically numbered using the IMGT Unique Numbering scheme, anchoring at Cys23, Trp41, Leu89, and Cys104.

>humIGHV3-23_AA Canonical_Human_Framework
EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSS

Raw Sequence Intake: You can paste raw unformatted text directly into the box. Whitespace, numbers, FASTA headers, line breaks, and trailing spaces are automatically cleansed by WebAssembly.

EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSS

Ready for analysis

V-GENE & Allele

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D-GENE Match

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J-GENE & Boundary

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CDR3 & Lengths

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Productivity Status

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📿 IMGT Collier de Perles (2D Pearl Necklace)

Planar 2D structural layout published in Nucleic Acids Res. 1998 (Lefranc, Giudicelli, Busin, Bodmer, Müller et al. DOI: 10.1093/nar/26.1.297 ↗ | PMID: 9399859 ↗)

1. Alignment for V-GENE and Allele Identification

Bars (|) indicate identity; red indicates SHM mismatch

2. Results of IMGT/JunctionAnalysis Deconstruction

Decomposition into V, P, N1, D, N2, and J contributions

3. Collinear IMGT Unique Numbering Grid (Positions 1 to 128)

FR1 (1-26) | CDR1 (27-38) | FR2 (39-55) | CDR2 (56-65) | FR3 (66-104) | CDR3 (105-117) | FR4 (118-128)

4. V-REGION Protein Display (Chemical Property Colors)

Standard immunogenetic amino acid property stratification

Aliphatic/Hydrophobic (A,V,L,I,M)

Aromatic (F,Y,W)

Basic Positive (K,R,H)

Acidic Negative (D,E)

Polar Uncharged (S,T,N,Q)

Cysteine (Cys23/Cys104)

Proline

Glycine

5. V-REGION Mutation and AA Change Statistics

Silent (S) vs. Replacement (R) somatic hypermutation rates

Region	Nucleotide Length	Total Mutations	Silent (S)	Replacement (R)	R/S Ratio	Mutation Rate (%)